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IMMUNOHISTOCHEMICAL DETECTION OF P-GLYCOPROTEIN IN TELEOST TISSUES USING MAMMALIAN POLYCLONAL AND MONOCLONAL ANTIBODIES
Citation:
Hemmer, M., L. Courtney, AND L. Ortego. IMMUNOHISTOCHEMICAL DETECTION OF P-GLYCOPROTEIN IN TELEOST TISSUES USING MAMMALIAN POLYCLONAL AND MONOCLONAL ANTIBODIES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/372, 1995.
Description:
Mammalian P-glycoprotein is a highly conserved 170 kD integral plasma membrane protein functioning as an energy dependent efflux pump of exogenous and endogenous lipophilic aromatic compounds entering the cell by diffusion. n this study, the tissue specificity of one polyclonal (pAb) and three monoclonal (mabs) antibodies to mammalian P-glycoprotein were identified in paraffin-embedded, parasagittal whole body sections of the guppy, Poecilia reticulate. ab mdr (Ab- 1) and mabs C219, C494 and JSB-1 demonstrated differential staining patterns in the following tissues: bile canaliculi in the liver, exocrine pancreas, lumenal surface of the intestinal epithelium, renal tubules, interrenal tissue, branchial blood vessels, gas gland, pseudobranch, and the gill transverse septa. ositive P-glycoprotein expression in P. reticulate correlates well with published results for homologous mammalian tissues of secretory and excretory function. hese data indicate that one or more highly conserved members of the P-glycoprotein transporter family exist in a teleost species which can be detected using commercially available mammalian antibodies.