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DETERMINATION OF ACRYLAMIDE IN RAT SERUM AND SCIATIC NERVE BY GAS CHROMATOGRAPHY-ELECTRON-CAPTURE DETECTION
Citation:
Raymer, J., C. Sparacino, G. Velez, S. Padilla, R. MacPhail, AND K. Crofton. DETERMINATION OF ACRYLAMIDE IN RAT SERUM AND SCIATIC NERVE BY GAS CHROMATOGRAPHY-ELECTRON-CAPTURE DETECTION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/070 (NTIS PB94141587), 1993.
Description:
A modified method for the derivatization and analysis of acrylamide as 2-bromopropenamide by gas chromatography/electron capture detection was validated in serum and sciatic nerve from rats. he method was accurate and precise over the concentration range of 2240 to 74700 ppm (w/v) in serum diluted 1:125 and 4 to 122 ppm (w/w) in sciatic nerve tissue present at 5 mg/ml. imits of detection were estimated to be 3 ppm in intact sciatic nerve and 1200 ppm in undiluted serum. he use of less dilute samples to allow for lower limits of detection appears feasible. he time-course of acrylamide in serum and sciatic nerve was studied after acute dosing and indicated elimination half-lives of 1.8 and 2.0 hours for serum and sciatic nerve, respectively. ose-response relationship was established for each matrix after acute dosing and the measured acrylamide concentrations in serum were approximately 1000 fold higher than in sciatic nerve.