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CHARACTERIZATION OF CATALASE ACTIVITIES IN A ROOT-CLEANING ISOLATE OF PSEUDOMONAS PUTIDA
Citation:
Katsumon, J. AND A. Anderson. CHARACTERIZATION OF CATALASE ACTIVITIES IN A ROOT-CLEANING ISOLATE OF PSEUDOMONAS PUTIDA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/222 (NTIS PB93205052), 1992.
Description:
Psuedomonas putida, a saprophytic root-colonizing bacterium, produces multiple forms of catalase Catalase A which increases in specific activity during growth phase and after treatment with H2O2, is located in the and is inhibited by 3-amino-1,2-4-triazole, EDTA, and cyanide, but not by chloroform-methanol treatment, which is induced by external H2O2 or during stationary phase of growth, is membrane associated by chloroform-methanol, EDTA, and cyanide, but not by aminotriazole. atalase A has a broad pH optimum by ethyl methanesulfonate mutagenesis, lacked catalase A activity in extracts of cells harvested throughout lag to earn stationary growth phase in liquid medium. atalase B was produced by J-1 in stationary phase. xposure of the wild type upon direct exposure to 2.5 mM H2O2 but survived this treatment after exposure to lower 10.3 MM, nonlethal doses of H2O2. he ability to adapt to H2O2 may be related to the behavior of J-1 on roots where active oxygen species are produced by root surface enzymes. -1 colonized root surfaces at wild-type levels and produced catalases A and B after exposure to root surfaces for 12 h.
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CHARACTERIZATION OF CATALASE ACTIVITIES IN A ROOT-CLEANING ISOLATE OF PSEUDOMONAS PUTIDA