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PHARMACOLOGIC PROBING OF RENAL DEVELOPMENT IN THE NEONATAL RAT
Citation:
Gray, J. AND R. Kavlock. PHARMACOLOGIC PROBING OF RENAL DEVELOPMENT IN THE NEONATAL RAT. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-86/312 (NTIS PB87178810).
Description:
The study was designed to examine the ontogeny of renal functions in the neonatal rat using various pharmacologic agents as probes. The renal responses of 2, 6, and 10 day old rats to diuretic agents known to act on proximal tubules, loops of Henle and distal tubules were assessed. These included acetazolamide, furosemide, mercaptomerin, chlorothiazide and amiloride. Acetazolamide, furosemide, chlorothiazide and amiloride induced diuresis at each age indicating that the respective reabsorptive mechanisms were present and functional by 2 days of age. At all ages furosemide evoked a maximal response in eliminating the interstitial fluid gradient as indicated by the formation of an isosmotic urine in treated pups. However, the volume of the diuresis at 2 days of age was half of those at 6 and 10 days, reflecting enhanced activity of the countercurrent multiplication apparatus in the maturing pups. Administration of mercaptomerin, did not produce a pharmacologic diuresis, but rather resulted in acute renal failure; although the nephrotoxicity was to a lesser extent in the two day old pups.