You are here:
OXIDATION OF NITROPYRIN TO 6-CHOLORPICOLINIC ACID BY THE AMMONIA-OXIDIZING BACTERIUM NOSTROSOMAS EUROPAEA
Citation:
Vannelli, T. AND A. Hooper. OXIDATION OF NITROPYRIN TO 6-CHOLORPICOLINIC ACID BY THE AMMONIA-OXIDIZING BACTERIUM NOSTROSOMAS EUROPAEA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/073.
Description:
Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. apid oxidation of nitrapyrin (at a concentration of 10 uM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. he turnover rate was highest in the presence of 10 mM ammonia (0.8 nmol of nitropyrin per min/mg of protein). he product of the reaction was 6-chloropicolinic acid. y the use of 18O2, it was shown that one of the oxygens in 6-chloropicolinic acid came from diatomic oxygen and radioactivity of [2,6-14C]nitrapyrin was shown to bind to cells. ost (94%) of the latter was bound indiscriminately to membrane proteins. he nitrapyrin bound to membrane proteins may account for the observed inactivation of ammonia oxidation.