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COMPARATIVE YIELDS OF MUTAGENS FROM CIGARETTE SMOKERS' URINE OBTAINED BY USING SOLID-PHASE EXTRACTION TECHNIQUES
Citation:
Williams, R., T. Pasley, R. Watts, J. Inmon, J. Fitzgerald, AND L. Claxton. COMPARATIVE YIELDS OF MUTAGENS FROM CIGARETTE SMOKERS' URINE OBTAINED BY USING SOLID-PHASE EXTRACTION TECHNIQUES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/223.
Description:
Urine from cigarette smokers was prepared for mutagenicity testing by extracting mutagens with solid phase extraction columns. ommercially available prepacked bonded silicas (cotadecyl, cyclohexyl, cyanopropyl) were compared for their efficiency and specificity in concentration of the urinary mutagens. ecovered urinary metabolites were evaluated for mutagenic activity using a micro reversion assay with Salmonella typhimurium. ose response data indicated that while mutagens were recovered by all three adsorbents, samples prepared using the bonded cyanopropyl columns yielded the most bioactivity and/or the least amount of test organism toxicity. arying pH of the urine was found to influence only the basified samples in terms of mutagenic recovery with the cyanopropyl phase. ombinations of different adsorbents were not found to offer significant advantages over use of a singular extraction adsorbent.