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BIODEGRADATION OF DDT [1,1,1-TRICHLORO-2,2-BIS)4-CHLOROPHENYL) ETHANE] BY THE WHITE ROT FUNGUS PHANEROCHAETE CHRYSOSPORIUM
Citation:
Bumpus, J. AND S. Aust. BIODEGRADATION OF DDT [1,1,1-TRICHLORO-2,2-BIS)4-CHLOROPHENYL) ETHANE] BY THE WHITE ROT FUNGUS PHANEROCHAETE CHRYSOSPORIUM. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/164.
Description:
Extensive biodegradation of 1,1,1-trichloro-2,2bis(4-chlorophenyl) ethane (DDT) by the white rot fungus Phanerochaete chrysosporium was demonstrated by disappearance and mineralization of [14C]DDT in nutrient nitrogen-deficient cultures. ass balance studies demonstrated the formation of polar and water-soluble metabolites during degradation. exane-extractable metabolites identified b,y gas chromatography-mass spectrometry included 1,1-dichloro-2,2-bis(4-chlorophenyl)ethane (DDD), 2,2,2-tri- chloro-1,1-bis(4-chlorophenyl)ethanol (dicofol), 2,2-dichloro-1,1-bis(4-chlorophenyl) ethanol (FW-152), and 4,4'-dichlorobenzophenone (RBP). DD was the first metabolite observed; it appeared after 3 days of incubation and disappeared from culture upon continued incubation. his, as well as the fact that [14C]dicofol was mineralized, demonstrates that intermediates framed during DDT degradation are also metabolized. hese results demonstrate that the pathway for DDT degradation in P. chrysosporium is clearly different from the major pathway proposed for microbial or environmental degradation of DDT. ike P. chrysosporium ME-446 and BKM-F-1767, the white rot fungi Pleurotus ostreatus, Phellinus weirii, and Polyporus versicolor also mineralized DDT.