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SYNAPTONEMAL COMPLEX DAMAGE AS A MEASURE OF CHEMICAL MUTAGEN EFFECTS ON MAMMALIAN GERM CELLS
Citation:
Allen, J.W., G. DeWeese, J. Gibson, P. Poorman, AND M. Moses. SYNAPTONEMAL COMPLEX DAMAGE AS A MEASURE OF CHEMICAL MUTAGEN EFFECTS ON MAMMALIAN GERM CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-87/028 (NTIS PB87198461).
Description:
As heritable chromosome anomalies are implicated in a variety of human disabilities, their induction in germ cells by environmental chemicals is viewed as a threat to health. Synaptonemal complex (SC) analysis is a novel approach for the detection of germ-line chromosomal damage. This sensitive cytological procedure reveals induced structural damage and pairing abnormalities in SCs of meiotic prophase chromosomes, together with other germ-line toxic effects, in the testes of rodents treated with mitomycin C and cyclophosphamide. Our results demonstrate the effectiveness of SC analysis as a rapid and practical in vivo germ-line mutagen assay that lacks many of the shortcomings of existing tests. (Copyright (c) 1987 Elsevier Science publishers B.V. Biomedical Division.)