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ECTOMYCORRHIZAL DNA: ISOLATION, RFLPS, AND PROBE HYBRIDIZATION
Citation:
Rygiewicz, P. AND J. Armstrong. ECTOMYCORRHIZAL DNA: ISOLATION, RFLPS, AND PROBE HYBRIDIZATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-90/017.
Description:
We are in a new era for research in terrestrial ecology. s with diagnostics and research in medicine and forensics, molecular biology is transforming the way we pose hypotheses, conceptualize experiments, and conduct research in rhizosphere ecology. any soil-borne organisms are morphologically indistinguishable, can change morphology from one sexual state to another, or are not culturable in vitro. hese properties hinder our understanding of rhizosphere structure and function. ontemporary, molecular-based methods to isolate DNA from individuals. he purpose of this chapter is to present a primer of basic methods that have direct application to recovery of DNA from mycorrhizal fungi in soils.