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32P-POSTLABELING DNA ADDUCT ASSAY: CIGARETTE SMOKE-INDUCED DNA ADDUCTS IN THE RESPIRATORY AND NONRESPIRATORY RAT TISSUES
Citation:
Gupta, R. AND C. Gairola. 32P-POSTLABELING DNA ADDUCT ASSAY: CIGARETTE SMOKE-INDUCED DNA ADDUCTS IN THE RESPIRATORY AND NONRESPIRATORY RAT TISSUES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-90/243.
Description:
An analysis of the tissue DNA adducts in rats by the sensitive 32P-postlabeling assay showed one to eight detectable DNA adducts in lung, trachea, larynx, heart and bladder of the sham controls. hronic exposure of animals to mainstream cigarette smoke showed a remarkable enhancement of most adducts in the lung and heart DNA. In contrast, four of the five nasal DNA adducts formed in the smoke-exposed rats were undetectable in control animals. Since cigarette smoke contains several thousand chemicals and a few dozen of them are known or potential carcinogens, the difference between the DNA adducts of nasal and the other tissues may reflect the diversity of reactive constituents and their differential absorption in different tissues. he smoke-related tissue DNA adducts were aromatic and/or hydrophobic in nature. n comparison to the lung DNA adducts, the adducts in nasal DNA were less hydrophobic. dentity of the predominant adducts was further investigated by comparison with several reference DNA adducts from 10 PAH and aromatic amines. Since some of these chemicals are present in cigarette smoke, our results suggest that these constituents of cigarette smoke may not be directly responsible for formation of DNA adducts In the lung and heart of the smoke-exposed animals.