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IN VITRO AUGMENTATION OF NATURAL KILLER CELL ACTIVITY BY MANGANESE CHLORIDE
Citation:
Smialowicz, R., R.R. Rogers, M. Riddle, D. Rowe, AND R. Luebke. IN VITRO AUGMENTATION OF NATURAL KILLER CELL ACTIVITY BY MANGANESE CHLORIDE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-86/286 (NTIS PB87175089).
Description:
The in vitro cultivation of murine spleen cells with MnCl2 resulted in the enhancement of natural killer (NK) cell activity as measured in a 4-h (51)Cr-release assay. Optimal enhancement of NK activity was observed at concentrations of 10-20 micrograms MnCl2 culture (72-144 micromolarity Mn(2+). Enhancement of NK activity by MnCl2 was not associated with any changes in the number or viability of cells following culture. The enhancement of NK activity by MnCl2 in vitro was accompanied by interferon induction. The results demonstrate that murine splenic NK activity is enhanced in vitro by MnCl2 and this enhancement may be mediated by interferon induction. The results also suggest that in vitro enhancement of NK activity by MnCl2 as with Poly 1 C, may require participation of an adherent cell population for NK augmentation.