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TOXICOLOGICAL EVALUATION OF COMPLEX INDUSTRIAL WASTES: IMPLICATIONS FOR EXPOSURE ASSESSMENT
Citation:
DeMarini, D.M., J. Gallagher, V. Houk, AND J. Simmons. TOXICOLOGICAL EVALUATION OF COMPLEX INDUSTRIAL WASTES: IMPLICATIONS FOR EXPOSURE ASSESSMENT. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/385 (NTIS PB90216144).
Description:
We evaluated a variety of short-term bioassays to construct a battery of tests that could be used for assessing the biological effects of potentially hazardous complex industrial wastes. en samples were studied for hepatotoxicity: hese samples and an additional five were studied for mutagenicity. lthough the data are limited to these samples, the results suggest that the Salmonella assay (either TA98 or TAlOO) or a prophageinduction assay (both in the presence of S9) bin combination with determination of relative liver weight and levels of a set of serum enzymes in rats would provide a battery of tests suitable to characterize complex industrial wastes for mutagenic and hepatotoxic potential. he biological activities exhibited by the wastes were not readily predicted by the chemical profiles of the wastes, emphasizing the importance of characterizing potentially hazardous complex industrial wastes by both chemical and biological means. NA from liver, lung, and bladder of rats exposed to some of the wastes was analyzed by the 32 P-postlabeling technique for the presence of DNA adducts. aste that produced mutagenic urine was found to produce a DNA adduct in bladder tissue DNA. he implications of this approach for assessment of exposure to complex hazardous waste mixtures are discussed.