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IDENTIFICATION OF ORGANIC N-CHLORAMINES IN VITRO IN STOMACH FLUID FROM THE RAT AFTER CHLORINATION
Citation:
Scully, Jr., F., K. Mazina, H. Ringhand, AND E. Chess. IDENTIFICATION OF ORGANIC N-CHLORAMINES IN VITRO IN STOMACH FLUID FROM THE RAT AFTER CHLORINATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/510.
Description:
When 36Cl-N-chloropiperidine (NCP)(300 mg/L as Cl2) was mixed with stomach fluid from laboratory rats (fasted either 24 or 48 hr and incubated in the dark for 30 min at 37 degrees C and at higher pH values, approximately one-third was reduced to chloride and between 7% and 63% of the radiolabel was found to chromatograph in a manner distinctly different from 36Cl-chloride of 36CL-NCP. This new fraction is referred to as the 36-Cl-chloroorganic fraction. he remainder of the radiolabel was associated with 36CL-NCP. arying concentrations of 36CL-NCP (3-200 mg/L as CL2) have incubated for 30 min at 37 degrees C with stomach fluid at varying pH on the product distribution. t low pH values a threshold concentration of approximately 100 mg/L active chlorine was measured below which all chloramine was reduced to 36CL-chloride. s the pH and concentration of 36CL-NCP were increased the percentage of chloramine reduced to chloride decreased. sing concentrations between 110 and 120 mg/L (CL2) 7% and 4% of the label was associated with the 36Cl-chloroorganic fraction at pH 7.1 and at pH 2.2, respectively.