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URBAN AEROSOL ACIDS: ANALYSIS OF NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY STANDARD REFERENCE MATERIAL 1649
Citation:
Sparacino, C., S. Frazier, M. Nishioka, AND J. Lewtas. URBAN AEROSOL ACIDS: ANALYSIS OF NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY STANDARD REFERENCE MATERIAL 1649. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/542 (NTIS PB91242529).
Description:
Urban air particulate matter, collected from Washington, D.C. and certified by the National Institute of Standards and Technology (NIST) as Standard Reference Material 1649, was extracted and fractionated into acid, base and neutral fractions. ach fraction was tested for biological activity using a microbial mutagenesis assay system. he organic acid fraction showed unexpectedly high mutagenic activity, and was subjected to chemical characterization studies. ollowing derivatization, analysis by CC/MS showed the presence of fatty acids, aromatic acids (including phenolic compounds), and a significant number of compounds that could not be identified from mass spectral compendia. pectroscopic and elemental analysis data supported the characterization of the fraction as predominantly aromatic. ass spectra from both CC/MS and direct probe analysis showed the presence of a chlorinated substance, subsequently identified as the fungicide Dichlorophen. he compound was shown to comprise over 50 percent of the mass of the organic acid fraction. eference standard of Dichlorophen was not mutagenic. he presence of the fungicide in the NIST certified urban aerosol is, in all probability due to artifactual processes. ttempts to concentrate the observed mutagenic activity by preparative chromatography and acid/base partition experiments were not successful.
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URBAN AEROSOL ACIDS: ANALYSIS OF NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY STANDARD REFERENCE MATERIAL 1649