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ANALYSIS OF CHLORINATED HERBICIDES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY
Citation:
Jones, T.L., L. Betowski, AND J. Yinon. ANALYSIS OF CHLORINATED HERBICIDES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-90/021 (NTIS PB90221698).
Description:
A method that uses high performance liquid chromatography/mass spectrometry (HPLC/MS) for the analysis of chlorinated phenoxyacid herbicides is described. uring method development different techniques were used to increase both the sensitivity and the specificity of thermospray HPLC/MS for chlorinated acid herbicides. These included the operation of the instrument in the negative chemical ionization (NCI) mode initiated by discharge and the use of a wire-repeller in the ion source for efficient extraction of positive ions. Single quadrupole repeller-induced and multiple quadrupole collision activated dissociation (CAD) experiments were also performed to increase the structural information of the mass spectra. This method complements the U.S. Environmental Protection Agency's gas chromatographic (GC) SW-846 Method 8151 for the determination of chlorinated acid herbicides. Method 8151 prescribes the use of hydrolysis to ensure that these compounds are in the acid form, reaction of the acids with diazomethane to produce methyl esters, and GC or GC/MS analysis. This HPLC method eliminates the need for most sample preparation steps and allows direct analysis of sample extracts.