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GUIDELINES FOR CONDUCTING EARLY LIFE STAGE TOXICITY TESTS WITH JAPANESE MEDAKA (ORYZIAS LATIPES)
Citation:
Benoit, D., G. Holcombe, AND R. Spehar. GUIDELINES FOR CONDUCTING EARLY LIFE STAGE TOXICITY TESTS WITH JAPANESE MEDAKA (ORYZIAS LATIPES). U.S. Environmental Protection Agency, Washington, D.C., EPA/600/3-91/063 (NTIS PB92137488).
Description:
This manual represents a procedural guide for conducting embryo-larval early life stage (ELS) toxicity tests with Japanese medaka (Oryzias latipes). hese procedures are based upon evaluation of published papers and recent methods development work conducted at our laboratory in Duluth. he published papers are referenced in the appropriate places throughout the text of this report. If more detailed information on test apparatus or specific biological and chemical methods is desired, the reader is encouraged to study the reference material or contact one of the authors of this manual. ll routine methods not covered in this procedure (physical and chemical determinations), should be followed as described in Standard Methods for the Examination of Water and Wastewater (3). hose routines dealing with handling of fish not covered in this procedure should be followed as described by ASTM (4).