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IDENTIFICATION OF ANEUPLOIDY-INDUCING AGENTS USING THE WHEAT SEEDLING ASSAY
Citation:
Sandhu, S., J. Dhesi, AND B. Gill. IDENTIFICATION OF ANEUPLOIDY-INDUCING AGENTS USING THE WHEAT SEEDLING ASSAY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-89/091.
Description:
A short-term assay using Chinese spring wheat for detecting induced aneuploidy and/or clastogenicity is described. he seed of virescent wheat (v1v1) are treated with a test agent. he appearance of white sectors (v1v1v1) or green sectors (v10) on the virescent leaves indicates the gain or loss of a chromosome or a segment of a chromosome bearing v1 allele. hree test chemicals, chromium trioxide (100-5,000 ug/ml), chromium dichromate (500-5,000 ug/ml), and cyclophosphamide (100-1,000 ug/ml) produced a dose related positive response for chromosome aberrations in this assay.