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DETECTION OF EXOGENOUS GENE SEQUENCES IN DISSOLVED DNA FROM AQUATIC ENVIRONMENTS
Citation:
DeFlaun, M. AND J. Paul. DETECTION OF EXOGENOUS GENE SEQUENCES IN DISSOLVED DNA FROM AQUATIC ENVIRONMENTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/174.
Description:
A method for the concentration and detection of gene sequences in the dissolved DNA from freshwater and marine environments has been developed. he limit of detection in the dot blot format was 167 fg/ml (100 ml sample) for exogenous herpes simplex thymidine kinase (TK) gene that was added to artificial seawater or river water. his procedure has been used to determine the longevity and monitor progressive changes in molecular weight of a plasmid containing the TK gene added to eutrophic estuarine water. he onset of plasmid degradation as determined by change in molecular weight was rapid (within 5 min). Intact plasmid was detected for at least 4 hours and sequences hybridizable to the TK gene probe were present for up to 24 hours.