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INHIBITION OF HUMAN NATURAL KILLER CELL ACTIVITY FOLLOWING IN VITRO EXPOSURE TO OZONE
Citation:
Harder, S., D. Harris, H. Koren, AND D. House. INHIBITION OF HUMAN NATURAL KILLER CELL ACTIVITY FOLLOWING IN VITRO EXPOSURE TO OZONE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/079 (NTIS PB90245473).
Description:
In this study we have examined the effect of in vitro ozone exposure on human peripheral blood natural killer (NK) cell activity measured against K562 tumor cells. he data showed that NK activity was nhibited in a time dependent manner with marked suppression observed after 6 hours of ozone exposure. ignificant inhibition occurred at three different levels of ozone exposure (1.0, 0.5 and 0.18 ppm) and E/T ratios of (50:1, 25:1 and 12.5:1). he ozone effect was not merely a nonspecific toxicity as NK activity recovered within 18 hours following exposure either spontaneously or by stimulation with IL 2. oreover, unexposed cells treated with supernatant from ozone exposed cells showed no decrease in NK activity. irect effect of ozone on the natural killer cells was suggested by the finding of suppressed activity in an NK enriched population. ince conjugate formation of the ozone exposed NK cells with K562 targets was unchanged we assume that the inhibitory effect of ozone was at a post binding step in the lytic pathway.