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CLONING AND EXPRESSION OF THE CATA AND CATBC GENE CLUSTERS FROM PSEUDOMONAS AERUGINOSA PAO
Citation:
Kukor, J., R. Olsen, AND D. Ballou. CLONING AND EXPRESSION OF THE CATA AND CATBC GENE CLUSTERS FROM PSEUDOMONAS AERUGINOSA PAO. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/529.
Description:
A 9.9-kilobase (kb) BAMIII estriction endonuclease fragment encoding the catA and catBC gene clusters was selected from a gene bank of the Pseudomonas aeruginosa PAO1c chromosome. he catA, catB, and catC genes encode enzymes that catalyze consecutive reactions in the catechol branch of the beta-ketoadipate pathway. ecombinant plasmid, pRol783, which contains the 9.9-kb BAMIII restriction fragment complemented P. aeruginosa, mutants with lesions in the catA, catB, or catC gene; however, this fragment of chromosomal DNA did not contain any other catabolic gene which had been placed near the catA or catBC cluster based on cotransducibility of the loci. estriction mapping, deletion subcloning, and complementation analysis showed that the order of the genes on the cloned chromosomal DNA fragment is catA, catB, catC. hese observations suggest positive regulation of the P. aeruginosa and cluster, the absence of a positive regulatory element from pR0l783 and the inability of the P. putida regulatory gene product to induce expression of the P. aeruginosa and catA, catB, and catC genes.