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NEUTOPHIL MYELOPEROXIDASE DESTRUCTION BY ULTRAVIOLET IRRADIATION
Citation:
Hanker, J., B. Giammara, AND G. Strauss. NEUTOPHIL MYELOPEROXIDASE DESTRUCTION BY ULTRAVIOLET IRRADIATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/508.
Description:
The peroxidase activity of enriched leukocyte preparations on coverslips was determined cytochemically with our newly developed methods. his technique utilizes diaminobenzidine medium and cupric nitrate intensification and is suitable for analysis with light microscopy, SEM, and TEM. lood specimens from control individuals were studied with and without in vitro UV irradiation and compated with those from prosiasis patients exposed therapeutically to various types of UV in phototherapy. ll UV irradiated samples showed diminished neutrophil myeloperoxidase phototherapy. ll UV irradiated samples showed diminished neutrophil myeloperoxidse (MP) activity although that of the principal eosinophil peroxidase was unaffected. he SEMs supported the contention that decreased neutrophil MP activity might be related to UV induced degranulation. e believe it will be possible, eventually, to equate the observed MP degranulation effect after UV irradiation with diminished ability to fight bacterial infections.