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CYTOGENETIC STUDIES OF MICE EXPOSED TO STYRENE BY INHALATION
Citation:
Kligerman, A., J.W. Allen, M. Bryant, J. Campbell, B. Collins, C.L. Doerr, G. Erexson, P. Kwanyuen, AND D. Morgan. CYTOGENETIC STUDIES OF MICE EXPOSED TO STYRENE BY INHALATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/403 (NTIS PB93236404).
Description:
The published data for the in vivo genotoxicity of styrene (STY) are equivocal. o evaluate the clastogenicity and sister chromatid exchange (SCE)-inducing potential of STY in vivo under carefully controlled conditions, 36C3F1 female mice were exposed by inhalation for 6 hours/day for 14 consecutive days to either 0, 125, 250, or 500 ppm STY. ne day after the final exposure, peripheral blood, spleen, and lungs were removed and cells were cultured for the analysis of micronucleus (MN) induction using the cytochalasin B-block method, chromosome breakage, and SCE induction. eripheral blood smears were also made for scoring MN in erythrocytes. here was a significant elevation of SCE frequency in lymphocytes from the spleen and the peripheral blood as well as in cells from the lung. owever, no statistically significant concentration-related increases were found in the frequency of chromosome aberrations in the cultured splenocytes or lung cells, and no significant increases of MN were observed in binucleated splenocytes or normochromatic erythrocytes in peripheral blood smears.