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IPCS COLLABORATIVE STUDY ON PLANT SYSTEMS TO DETECT GENOTOXIC EFFECTS OF ENVIRONMENTAL CHEMICALS: VICIA FABA CHROMOSOMAL ABERRATION ASSAY
Citation:
Kanaya, N., B. Gill, I. Grover, A. Murin, R. Osiecka, S. Sandhu, AND H. Anderson. IPCS COLLABORATIVE STUDY ON PLANT SYSTEMS TO DETECT GENOTOXIC EFFECTS OF ENVIRONMENTAL CHEMICALS: VICIA FABA CHROMOSOMAL ABERRATION ASSAY. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/534 (NTIS PB95148961).
Description:
A collaborative study involving laboratories in six countries was initiated under the auspices of the International Programme on Chemical Safety (IPCS), the United Nations Environment Programme (UNEP), the International Labour Organization (ILO) and the World Health Organization (WHO) to determine the sensitivity, efficiency and reliability of the Vicia faba root-tip meristem chromosomal aberration assay using a standardized protocol. he six laboratories that participated in this study were located in Czechoslovakia, india, Japan, Poland, Sweden and the U.S.A. All laboratories adhered to a standardized protocol for the Vicia faba chromosomal aberration assay. our coded chemicals, azidoglycerol (AG), methyl nitrosourea (MNU) , sodium azide (NaN3) , and maleic hydrazide (MH) were tested with the Vicia faba chromosomal aberration assay. f the 4 chemicals, 3 (MH, AG and MNU) were found to be clastogenic and gave a concentration-related response. owever, the results for NaN3 were equivocal, which might be explained by the stability of NaN, The conclusions from this study suggest that the Vicia faba chromosomal aberration bioassay is an efficient and reliable short-term bioassay for the rapid screening of chemicals for clastogenicity.