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PREDICTIVE MODEL OF CONJUGATIVE PLASMID TRANSFER IN THE RHIZOSPHERE AND PHYLLOSPHERE
Citation:
Knudsen, G., M. Walter, L. Porteous, V. Prince, J. Armstrong, AND R. Seidler. PREDICTIVE MODEL OF CONJUGATIVE PLASMID TRANSFER IN THE RHIZOSPHERE AND PHYLLOSPHERE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-88/297 (NTIS PB89202329).
Description:
A computer simulation model was used to predict the dynamics of survival and conjugation of Pseudomonas cepacia (carrying the transmissible recombinant plasmid R388:Tn1721) with a nonrecombinant recipient strain in simple rhizosphere and phyllosphere microcosms. lasmid transfer rates were derived for a mass action model, and donor and recipient survival were modeled as exponential growth and decay processes or both. ate parameters were derived from laboratory studies in which donor and recipient strains were incubated in test tubes with a peat vermiculite solution or on excised radish or bean leaves in petri dishes. he model predicted donor, recipient, and transconjugant populations in hourly time Isteps. t was tested in a microcosm planted with radish seeds and inoculated with donor and recipient strains and on leaf surfaces of radish and bean plants also growing in microcosms. acteria were periodically enumerated on selective media over 7 to 14 days. hen donor and recipient populations were 1O-1 to 10-8 CFU/4g (wet weight) of plant or soil, transconjugant populations of about 10-1 to 10-4 were observed after 1 day. n initial rapid increase and a subsequent decline in numbers of transconjugants in the rhizosphere and on leaf surfaces were correctly predicted.