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FIBER OPTIC FLUOROIMMUNOSENSOR FOR THE DETECTION OF POLYCHLORINATED BIPHENYLS
Citation:
Rogers, K., J. Emon, R. Kline, J. Wright, Q. Zhao, AND E. Eldefrawi. FIBER OPTIC FLUOROIMMUNOSENSOR FOR THE DETECTION OF POLYCHLORINATED BIPHENYLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/A-95/058.
Description:
There currently exists a critical and growing need for cost-effective, real-time, and in situ methods for detection of environmental pollutants such as polychlorinated biphenyls (PCBs). erein reported is a evanescent wave Fluoroimmunosensor for measurement of PCBS. 2,4-5-Trichlorophenoxybutyric acid (TCPB) was cross linked to fluorescein ethyleneimine (TCPB-FL) and used as an optical signal generator. rotein A purified IgG, obtained from anti-PCB serum, was immobilized on quartz fibers and used as the biological recognition element in an evanescent wave fluorimeter. he optical signal was generated by the binding of the fluorescent probe TCPB-FL to the antibody (Ab) coated fiber. luorescent probe concentrations in the nM range resulted in reversible binding. everal Aroclors displaced the fluorescent probe (TCPB-FL) in a concentration-dependent manner. roclor 1248 was detected from the ppb to ppm range. roclor 1242 was also measured in methanol extracts from standard reference material soils.