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FIELD CALIBRATION OF SOIL-CORE MICROCOSMS FOR EVALUATING FATE AND EFFECTS OF GENETICALLY ENGINEERED MICROORGANISMS IN TERRESTRIAL ECOSYSTEMS
Citation:
Bolton, H., J. Fredrickson, S. Bentjen, D. Workman, S. Li, AND J. Thomas. FIELD CALIBRATION OF SOIL-CORE MICROCOSMS FOR EVALUATING FATE AND EFFECTS OF GENETICALLY ENGINEERED MICROORGANISMS IN TERRESTRIAL ECOSYSTEMS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/3-91/041.
Description:
Pacific Northwest Laboratory compared intact soil-core microcosms and the field for ecosystem structural and functional properties after the introduction of a model genetically engineered microorganism (GEM). This project used two distinct microbial types as model GEMs, Gram negative Pseudomonas sp RC1, which was an aggressive root colonizer, and Gram-positive Streptomyces lividans TK24. he model GEMs were added to surface soil in separate studies throughout a ten month period. lso, RC1 was used in studies conducted during two consecutive field seasons to determine how year-to-year field variability influenced the calibration of microcosms with the field. he main conclusions of this research were that intact soil-core microcosms can be useful to simulate the field for studies of microbial fate and effects on ecosystem structural and functional properties. etter controls of environmental variables including temperature and moisture will be necessary to more closely simulate the field far future use of microcosms for risk assessment.