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ENCLOSED AQUATIC MULTIPSECIES TEST SYSTEM FOR TESTING MIBROBIAL PEST CONTROL AGENTS WITH NON-TARGET SPECIES
Citation:
Lightner, D., R. Thurman, AND T. Trumper. ENCLOSED AQUATIC MULTIPSECIES TEST SYSTEM FOR TESTING MIBROBIAL PEST CONTROL AGENTS WITH NON-TARGET SPECIES. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/4-89/027.
Description:
An enclosed test system was developed in which multiple species of aquatic animals and plants were tested experimentally for adverse non-target effects of wild-type and genetically altered microbial pest control agents (MPCAs). our different types of model MPCAs were tested in the multispecies system. hese included two different strains of the mosquito pathogen Bacillus sphaericus, a strain of Pseudomonas putida (used as a model for the genus), and the insect baculovirus AcMNPV. he fate, persistence, and infectivity of these model MPCAs were evaluated using traditional microbiological and histological methods, as well as specific microbiological assays for model MPCAs that were altered by addition of a unique genetic marker. or two of the model MPCAs, gene probes were used as a detection method to track the MPCA in the test system water and NTOs.