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CONTINUOUS-FLOW METHOD FOR MEASURING EFFECTS OF CHEMICALS ON SOIL NITRIFICATION
Citation:
Rhodes, A. AND C. Hendricks. CONTINUOUS-FLOW METHOD FOR MEASURING EFFECTS OF CHEMICALS ON SOIL NITRIFICATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/008 (NTIS PB90219668).
Description:
Because the productivity of terrestrial ecosystems is directly related to microbial nutrient cycling, understanding the effects of chemical contaminants on soil microbial processes is important. his study examined the effects of two model chemicals - Roundup (glyphosate) and N-Serve (nitrapyrin) - on nitrifying organisms in static, perfusion, and continuous-flow culture systems. xperimental concentrations were approximately 1, 10, and 100 X the spot application rate. oth products were shown to inhibit nitrification in the treated soils. oundup significantly reduced nitrification at 6.8 and 68 mg g-1 dry soil. -Serve completely inhibited nitrification at levels greater than 42 ug-1 dry soil in all cultural methods. n comparative studies with static batch and perfusion culture techniques, the continuous-flow system proved to be both reliable and useful in the culture of nitrifying bacteria. his method provides an alternative to traditional culture techniques in measuring chemical effects on microbial geochemical cycles and provides a new method for use in toxicity testing.
