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SURVIVAL AND ENUMERATION OF AEROSOLIZED AND FREEZE-DRIED GENETICALLY ENGINEERED E. COLI, UNDER CONTROLLED ENVIRONMENTAL CONDITIONS
Citation:
Israeli, E., B. Shaffer, AND B. Lighthart. SURVIVAL AND ENUMERATION OF AEROSOLIZED AND FREEZE-DRIED GENETICALLY ENGINEERED E. COLI, UNDER CONTROLLED ENVIRONMENTAL CONDITIONS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/414.
Description:
Aerosol survival of a genetically engineered strain of Escherichia coli demonstrated a more rapid die-off (i.e., death rate) compared to its parental wildtype. p to 77% of a freeze-dried and air-exposed genetically engineered microorganism (GEM) and wildtype bacteria could be resuscitated by the amendment of the dilution medium with catalase or bovine serum albumin (BSA). ehydration temperature was found to effect survival of freeze-dried and air-exposed E. coli, cold shock and rehydration caused 97%, loss in viability, while trehalose treatment was found to enhance survival by 5 to 10 fold for the wildtype and GEM, respectively.