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CO-OCCLUSION AND PERSISTENCE OF A BACULOVIRUS MUTANT LACKING THE POLYHEDRIN GENE
Citation:
Hamblin, M., N. Beek, P. Hughes, AND H. Wood. CO-OCCLUSION AND PERSISTENCE OF A BACULOVIRUS MUTANT LACKING THE POLYHEDRIN GENE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/034.
Description:
A co-occlusion process was evaluated as a commercially and ecologically acceptable strategy for the development of genetically improved baculovirus insecticides. oinfection of Spodoptera frugiperda (IPLB-SF-21) tissue culture cells with Aucographa californica nuclear polyhedrosis virus (AcMNPV) and an AcE10 mutant (AcE10) lacking the polyhedrin gene resulted in occlusion of both virus types within polyhedra. he amount of occluded AcE10 virions in progeny polyhedra populations during serial passage in Trichoplusia ni larvae was evaluated. aintenance of the mutant in progeny polyhedra rehired polyhedra inocula containing equal numbers of the two virus types at a high dose. ignificant reduction in occluded mutant nucleocapsids occurs with inoculum levels below a 100% lethal dose. t inoculum levels below a 30% lethal dose, the majority of fourth-instar larvae were infected with only one type of virus. he commercial application and ecological advantages of the co-occlusion process are discussed.