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DOSE DEPENDENCE OF ACETYLCHOLINESTERASE ACTIVITY IN NEUROBLASTOMA CELLS EXPOSED TO MODULATED RADIOFREQUENCY RADIATION
Citation:
Dutta, S., K. Das, B. Ghosh, AND C. Blackman. DOSE DEPENDENCE OF ACETYLCHOLINESTERASE ACTIVITY IN NEUROBLASTOMA CELLS EXPOSED TO MODULATED RADIOFREQUENCY RADIATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-94/022.
Description:
Radiofrequency radiation (RFR) at 915 and at 147 MHz, when sinusoidally amplitude modulated (AM) at 16 Hz, has been shown to enhance release of calcium ions from neuroblastoma cells in culture. he dose response is unusual, consisting of two power density 'windows' in which enhanced efflux occurs, separated by power density regions where no effect is observed. imilar result has also been reported for a chicken brain-tissue preparation. o explore the physiological importance of these findings, we have examined the impact of RFR exposure on a membrane-bound enzyme, acetylcholinesterase (AChE), which is intimately involved with the acetylcholine (ACh) neurotransmitter system. euroblastoma cells (NG108), exposed for 30 min to 147MHz radiation, AM at 16 Hz, demonstrated enhanced ACHE activity, as assayed by a procedure using 14C-labeled ACh. nhanced activity was observed only within a narrow time window between 7.0 and 7.5 hr after the cells were plated, and only when the exposure occurred at power densities identified in a previous report as being effective for altering the release of calcium ions. hus RFR affects both calcium ion release and AChE activity in nervous-system-derived cells in culture in a common dose-rate-dependent manner.