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DNA SORPTION TO SOILS AND SEDIMENTS
Citation:
Ogram, A., G. Sayler, D. Gustin, AND R. Lewis. DNA SORPTION TO SOILS AND SEDIMENTS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/038.
Description:
Deoxyribonucleic acid (DNA) adsorption of five soils, an acid-washed sand, and a lake sediment was investigated. ll DNA at environmentally relevant concentrations was adsorbed by soils containing a significant amount of montmorillonite at low to neutral pk values. tudies on the effects of DNA molecular size on adsorption to sand and a sandy soil were described by the Freundlich isotherm model (r2 >0.85), and revealed that the higher the molecular weight, the more the adsorption. he effects of ionic strength (as sodium phosphate buffer) on adsorption showed that adsorption decreases as [P04=) increases. rganic carbon was found to plan a relatively minor role in the adsorption of DNA to these soils. cheme for the extraction of DNA from soils was also developed.