You are here:
USE OF CYANOPROPYL-BONDED HPLC COLUMN FOR BIOASSAY-DIRECTED FRACTIONATION OF ORGANIC EXTRACTS FROM INCINERATOR EMISSIONS
Citation:
DeMarini, D.M., R. Williams, L.R. Brooks, AND M. Taylor. USE OF CYANOPROPYL-BONDED HPLC COLUMN FOR BIOASSAY-DIRECTED FRACTIONATION OF ORGANIC EXTRACTS FROM INCINERATOR EMISSIONS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/022 (NTIS PB93158863).
Description:
The present study has shown that cyanopropyl-(CN) bonded silica HPLC columns are applicable for the fractionation of mass and mutagenic activity of organic extracts from some incinerator emissions. ichloromethane-extractable organics from particles emitted by two different municipal waste incinerators and by a pilot-scale rotary kiln incinerator that was combusting polyethylene were fractionated by HPLC, and the mutagenicity of the fractions was determined by means of a microsuspension mutagenicity assay with Salmonella TA98. he CN-bonded silica columns provided high (80-100%) mass and mutagenicity recoveries for most emission extracts, and it fractionated the mutagenic activity. he results suggest that the emissions from municipal waste incinerators contain a high amount of direct-acting (-S9) mutagenic activity that is resolvable by HPLC using CN-bonded silica, Sub-fractionation of selected mutagenic HPLC fractions and subsequent analysis by gas chromatography/mass spectroscopy can be used to identify mutagenic species within complex incinerator emissions. he coupling of microsuspension bioassays to HPLC fractionation should be a useful tool for this type of analysis.