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RESTRICTION FRAGMENT LENGTH POLYMORPHISMS DISTINGUISH ECTOMYCORRHIZAL FUNGI
Citation:
Armstrong, J., N. Fowles, AND P. Rygiewicz. RESTRICTION FRAGMENT LENGTH POLYMORPHISMS DISTINGUISH ECTOMYCORRHIZAL FUNGI. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/112 (NTIS PB90108432).
Description:
Basidiomycetous fungi, two saprophytes and three mycorrhizal, were used to assess the specificity of DNA hybridization for distinguishing genera from one another. nterspecific comparisons were done with several isolates of mycorrhizal fungi, Laccaria bicolor and L. laccata, collected from diverse geographical sites. he DNAs were digested with four restriction nucleases and separated by gel electrophoresis into patterns of DNA fragments called restriction fragment length polymorphisms (RFLPs). he RFLPs were hybridized with a radioactively-labeled DNA probe encoding Basidiomycetous ribosomal RNA genes. he five genera were discernable using both unprobed and probed RFLPs. ybridization of probe DNA with RFLPs was isolate-specific for all nine Laccaria isolates examined. he reclassification of a L. bicolor isolate is supported, demonstrating that hybridization of RFLPs offers an additional tool for taxonomy of ectomycorrhizal fungi. he method may have field application for distinguishing known isolates if their DNA fingerprints are previously ascertained and are distinct from RFLPs of idigenous organisms