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REOVIRUSES IN WATER POLLUTION TESTING
Citation:
Spendlove, R., B. Barnett, D. George, D. Adams, AND D. Ridinger. REOVIRUSES IN WATER POLLUTION TESTING. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/1-84/022.
Description:
Twelve cell lines were tested to determine their sensitivity to reoviruses of three serotypes that had been isolated from sewage. Madin-Darby bovine kidney (MDBK) cells were the most susceptible. Sewage-isolated, protamine-precipitated reoviruses were also used in conjunction with MDBK cells in a comparative evaluation of immunofluorescent cell count (ICC) and plaque assay procedures. Immunofluorescence assay is more sensitive and more rapid than plaque assay procedure for quantifying reoviruses recovered from sewage. A procedure for protamine sulfate precipitation of reoviruses and rotaviruses was developed. Some reoviruses recovered from sewage were inactivated by enzyme treatment. This report describes a study to evaluate reoviruses for their potential usefulness in water quality testing.