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DYNAMIC INTERACTIONS OF PSEUDOMONAS AERUGINOSA AND BACTERIOPHAGES IN LAKE WATER
Citation:
Ogunseitan, O., G. Sayler, AND R. Miller. DYNAMIC INTERACTIONS OF PSEUDOMONAS AERUGINOSA AND BACTERIOPHAGES IN LAKE WATER. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/369.
Description:
The persistence and interaction between newly isolated strains of Pseudomonas aeruginosa and resident bacteriophages indigenous to a freshwater environment was monitored over 45 days in lake water microcosms. he interaction between susceptible and resistant bacteria with pure phage (UT1) particles or a mixed phage population (M1) was investigated by following temporal changes in host density, phage-to-bacteria ratio (PBR), and the appearance of apparent prophage carriers within the host population. ecay rates of the phage (UT1) ranged from 0.054 hour-1 in natural water to 0.027 hour in natural water to 0.027 hour in filtered lake water. About 45% of sensitive bacteria incubated with phase UT1 were pseudolysogenic within 12 hours of incubation in natural lake water. hage UT1 appeared to stabilize the density of host bacteria in lake water at a level of 10 colony-forming units (cfu)1. acterial coexistence with the mixed phage (M1) population resulted in an oscillating equilibrium with the PBR stabilizing at about 3. he presence of extraneous homoimmune phages appeared to be detrimental to the stability of the pseudolysogens, which were maintained at a lower population density than prophage-free cells in lake water containing the mixed phage (Ml) population.