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CLONING AND CHARACTERIZATION OF THE 'CL' REPRESSOR OF 'PSEUDOMONAS AERUGINOSA' BACTERIOPHAGE D3: A FUNCTIONAL ANALOG OF PHAGE LAMBDA 'C'I PROTEIN
Citation:
Miller, R. AND T. Kokjohn. CLONING AND CHARACTERIZATION OF THE 'CL' REPRESSOR OF 'PSEUDOMONAS AERUGINOSA' BACTERIOPHAGE D3: A FUNCTIONAL ANALOG OF PHAGE LAMBDA 'C'I PROTEIN. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-87/334 (NTIS PB88199419).
Description:
The authors cloned the gene (c1) which encodes the repressor of vegetative function of Pseudomonas aeruginosa bacteriophage D3. The cloned gene was shown to inhibit plating of D3 and the induction of D3 lysogens by UV irradiation. The efficiency of plating and prophage induction of the heteroimmune P. aeruginosa phage F116L were not affected by the presence of the cloned c1 gene of D3. When the D3 DNA fragment containing c1 was subcloned into pBR322 and introduced into Escherichia coli, it was shown to specifically inhibit the plating of phage lambda and the induction of the lambda prophage by mitomycin C. The plating of lambda imm(434) phage was not affected. (Copyright (c) 1987, American Society for Microbiology.)