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PERSISTENCE OF LYMPHOCYTES WITH DECENTRIC CHROMOSOMES FOLLOWING WHOLE-BODY X IRRADIATION OF MICE
Citation:
Kligerman, A., E. Halperin, G. Erexson, AND G. Honore. PERSISTENCE OF LYMPHOCYTES WITH DECENTRIC CHROMOSOMES FOLLOWING WHOLE-BODY X IRRADIATION OF MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/431 (NTIS PB91171868).
Description:
Thirty-six male C57B1/6 mice were whole-body x-irradiated with 3 Gy to generate lymphocytes with dicentric chromosomes to study the Persistence of these lymphocytes in the spleen and peripheral blood to estimate the lifespan of mature 8- and 7-cells. lood and spleen were removed from groups of four mice immediately after radiation exposure and on days 1, 3, 7, 14, 28, 56, and 112, thereafter. he initial frequencies of dicentric chromosomes with accompanying fragments observed in splenic 7-cells (0.44); splenic 8-cells (0.43), and peripheral blood lymphocyte cultures (0.48) initiated on day 0 were not significantly different. or both splenic and peripheral blood T-lymphocytes, the frequency of cells containing dicentric chromosomes declineD In an exponential manner following irradiation, with a 5-reduction in frequency occurring 14 days after exposure. n contrast, the frequency of B-cells containing dicentric chromosomes remained stable through day 7 but then declined precipitously between day 7 and 14 and remained relatively stable, although slightly above baseline, through day 112 post-exposure. or both B- and T-cells, less than 5% of the cells contained a dicentric chromosome with accompanying fragments at day 112. hese data Indicate that 8- and T-lymphocytes with dicentric chromosomes show different decay kinetics and suggest that they may possess different lifespans.