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SEQUENTIAL INOCULATION AS AN ADJUNCT IN ENTERIC VIRUS PLAQUE ENUMERATION
Citation:
Benton, W. AND C. Hurst. SEQUENTIAL INOCULATION AS AN ADJUNCT IN ENTERIC VIRUS PLAQUE ENUMERATION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-90/230 (NTIS PB91117085).
Description:
The potential utility of sequentially inoculating a virus sample onto two different cultures of similar dissimilar cell lines was evaluated in conjunction with IDU (5-iodo-2'-deoxyuridine) treatment of the cells as a potential adjunct in viral plaque formation assays. his evaluation was done using laboratory grown human echovirus 7, human enterovirus 69 and human poliovirus I. plus an environmental concentrate derived from sewage that contained indigenous untyped enteroviruses. he cell lines employed were BGM, RD, L-132 and HEL-299. equential inoculation generally yielded higher viral assay titers when compared with the more traditional method of simply introducing vital inoculum onto a culture of the first initial cell line and then completing the assay without removing that inoculum. hen a permissive cell line (BGM or RD) was used or the initial and final cultures in a sequential inoculation technique, the total plaque count titer from both the initial plus final cultures represented an average 35% improvement over the traditional method.