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INCREASED SUSCEPTIBILITY TO PENTOBARBITAL FOLLOWING MOUSE CYTOMEGALOVIRUS INFECTION: ROLE OF VIRAL-INDUCED INTERFERON
Citation:
Catignani, J., M. Menache, AND M. Selgrade. INCREASED SUSCEPTIBILITY TO PENTOBARBITAL FOLLOWING MOUSE CYTOMEGALOVIRUS INFECTION: ROLE OF VIRAL-INDUCED INTERFERON. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-89/460.
Description:
The purpose of this study was to determine the relative roles of viral induced interferon (IFN) and viral infection of the liver in mouse cytomegalovirus (MCMV)-induced depression of cytochrome P-450 (cyt P-450) levels and enhancement of pentobarbital-induced sleeping time (PEN-ST). his was done by establishing the temporal relationship among the IFN response, viral infection of the liver, suppression of cyt P-450 levels, and enhancement of PEN-ST, by determining the effect of anti-IFN antibody treatment on all of these responses, and by manipulating factors known to influence viral pathogenesis and host response to virus such as animal age, virulence of the virus, and dose of virus. n general, manipulation of these factors toward increased stimulation of host immune responses resulted in greater depression of cyt P-450. lthough IFN may be one of the factors involved, it does not appear to be the only factor responsible for the effects of MCMV infection on pentobarbital metabolism. he temporal relationship among the various responses measured following viral infection suggested that the effect of the IFN response may be indirect an due to modulation of other host defense mechanisms. se of anti-IFN antisera to definitively establish a role for IFN in the effects observed here proved unsuccessful. ffects of PEN-ST ad cyt P0-450 levels did not appear to be related to the magnitude of infection in the liver.