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RESPIROMETRIC MULTI-LEVEL BIODEGRADATION TESTING PROTOCOL ANDMETHODOLOGY FOR DETERMINING BIODEGRADATION KINETIC DATA
Citation:
Tabak, H., S. Desai, AND R. Govind. RESPIROMETRIC MULTI-LEVEL BIODEGRADATION TESTING PROTOCOL ANDMETHODOLOGY FOR DETERMINING BIODEGRADATION KINETIC DATA. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-90/140 (NTIS PB90264003).
Description:
Electrolytic respirometry involving natural sewage, sludge and soilmicrobiota is becoming prominent in fate studies of prioritypollutant and RCRA toxic organics to generatebiodegradation/inhibition kinetics data. he paper discusses theexperimental design and procedural steps for the respirometricbiodegradation and toxicity testing approach for individualorganics or specific industrial wastes at different concentrationlevels in a mineral salts medium. eveloped multi-level protocolis presented for determination of the biodegradability, microbialacclimation to toxic substrates and first order kinetic parametersof biodegradation for estimation of the Monod kinetic parameter oftoxic organic compounds, in order to correlate the extent and rateof biodegradation with a predictive model based on chemicalproperties and molecular structure of these compounds. Respirometric biodegradation/inhibition and biokinetic data areprovided for representative RCRA alkyl, chloro- and nitro-benzenes,phenols and selected CERCLA leachate toxic organics. ata on theeffects of the source of sludge biomass, temperature andconcentration of microbial inoculum on the kinetics ofbiodegradation are also included.