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INFLUENCE OF WATER QUALITY ON ENTERIC VIRUS CONCENTRATION BY MICROPOROUS FILTER METHODS
Citation:
Sobsey, M. AND J. Glass. INFLUENCE OF WATER QUALITY ON ENTERIC VIRUS CONCENTRATION BY MICROPOROUS FILTER METHODS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-84/093.
Description:
Four enteric viruses, poliovirus type 1, echovirus type 1, reovirus type 3 and simian adenovirus SV-11, were concentrated from seeded 1.3L-volumes of raw, finished and granular activated carbon (GAC)-treated waters by adsorption to 47 mm diameter (17 sq cm), electropositive (Virosorb 1MDS) filters at pH 7.5 or electronegative (Filterite) filters at pH 3.5 with and without 5 mM added MgCl2, followed by elution with 0.3% beef extract in 50 mM glycine at pH 9.5. Removal of particulates from raw and finished waters by 0.2 micrometer prefiltration prior to virus addition and pH adjustment had little effect on virus concentration efficiencies. Soluble organic compounds reduced virus adsorption efficiencies from both raw and finished waters compared to GAC-treated water, but the extent of interference varied with virus type and adsorption conditions. For electropositive 1MDS filters, organic interference was similar with all virus types. For Filterite filters, organic interference was evident with poliovirus and echovirus, but could be overcome by adding MgCl2. (Copyright (c) American Society for Microbiology 1984.)