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CO-CULTURE OF RAT EMBRYOS AND HEPATOCYTES: 'IN VITRO' DETECTION OF A PROTERATOGEN
Citation:
Oglesby, L., M. Ebron, B. Carver, R. Kavlock, AND P. Beyer. CO-CULTURE OF RAT EMBRYOS AND HEPATOCYTES: 'IN VITRO' DETECTION OF A PROTERATOGEN. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-86/070.
Description:
Rat embryos removed from the dam on day 10 of pregnancy were successfully co-cultivated in vitro with primary cultures of rat, rabbit, or hamster hepatocytes. Embryos co-cultivated with hepatocytes developed normally, as did embryos exposed to a test chemical, cyclophosphamide. In a system where embryos were co-cultivated with hepatocytes and then exposed to cyclophosphamide a dose-related embryotoxicity was observed, indicating metabolic activation of the proteratogen. When similar numbers of hepatocytes were used for metabolic activation of cyclophosphamide, the induced embryo-toxicity was greater in the presence of rabbit and hamster hepatocytes than with rat hepatocytes.