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STRAIN COMPARISONS OF DFB NEUROTOXICITY IN RATS
Citation:
Gordon, C. AND R. MacPhail. STRAIN COMPARISONS OF DFB NEUROTOXICITY IN RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/354 (NTIS PB93228609).
Description:
The purpose of this study was to assess intraspecies differences in behavioral and autonomic function in three strains of rat following administration of diisopropyl fluorophosphate (DFP), an irreversible inhibitor of acetylcholinesterase activity. ale rats of the Long-Evans (LE), Fischer 344 (F344), and Sprague Dawley (SD) strains were administered DFP at doses of 0 to 1.5 mg/kg (s.c.). The animals were placed 60 min later into one of two motor activity chambers and tested for 30 min. otor activity was measured using either a Doppler-based system or a commercial photocell device. ollowing measurement of motor activity in the Doppler system, body temperature (Tb) was measured and blood was then withdrawn by cardiac puncture and analyzed for serum cholinesterase activity (ChE). he remaining rats were retested 24 hr after DFP administration in the photocell device. he results showed a significant influence of strain on the effects of DFP. otor activity of LE rats was reduced by DFP at doses of 1.0 and 1.5 mg/kg, whereas the activity of F344 rats was reduced only at 1.5 mg/kg.