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A HUMAN BRONCHIAL EPITHELIAL CELL STRAIN WITH UNUSUAL IN VITRO GROWTH POTENTIAL WHICH UNDERGOES NEOPLASTIC TRANSFORMATION AFTER SV40 T ANTIGEN GENE TRANSFECTION
Citation:
Reddell, R., G. Stoner, I. Hsu, M. Mass, B. Hukku, A. Somers, B. Gerwin, S. Salghetti, A. Galati, W. Gunning, AND C. Harris. A HUMAN BRONCHIAL EPITHELIAL CELL STRAIN WITH UNUSUAL IN VITRO GROWTH POTENTIAL WHICH UNDERGOES NEOPLASTIC TRANSFORMATION AFTER SV40 T ANTIGEN GENE TRANSFECTION. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/255.
Description:
Bronchial epithelial cells were cultured from an individual with no evidence of malignant disease. hese cells, designated HB56B, had greatly extended in vitro life-span, being able to undergo 50 passages and 200 population doubling in contrast to the usual 3 to 4 passages and 20 to 30 population doubling characteristics of normal human bronchial epithelial cells. B56B cells had karyotypic evidence of an amplified region on the short arm of chromosome 11. nlike normal bronchial epithelial cells, which undergo terminal squamous differentiation in vitro in response to fetal bovine serum, kB568 cells were only minimally affected by serum. Those cells were readily established as an immortalized cell line. HB56B/5T, following transfection with a plasmid containing SV40 early region DNA. B56B cells were non-tumorigenic in thymic nude Ace, but HB56B/5T cells within a few passages of transfection with the SV40 plasmid formed tumors of which 28/37 regressed. B56B cells may offer an experimental system for the study of proliferation, differentiation, and senescence control in human bronchial epithelial cells.