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CIGARETTE SMOKE-INDUCED DNA ADDUCTS IN THE RESPIRATORY AND NONRESPIRATORY TISSUE OF RATS
Citation:
Gairola, C. AND R. Gupta. CIGARETTE SMOKE-INDUCED DNA ADDUCTS IN THE RESPIRATORY AND NONRESPIRATORY TISSUE OF RATS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-92/202.
Description:
Formation of DNA adducts is regarded a- an essential initial step in the process of chemical carcinogenesis. To determine how chronic exposure to cigarette smoke affects the distribution of DNA adducts In selected respiratory and nonrespiratory tissues, we exposed male Sprague-Dawley rats daily to fresh mainstream smoke from the University of Kentucky reference cigarettes (2Rl) in a nose-only exposure system for 32 weeks. lood carboxyhemoglobin, total particulate matter (TPM) intake, and pulmonary aryl hydrocarbon hydroxylase values indicated effective exposure of animals to cigarette smoke. NA was extracted from three respiratory (larynx,. trachea, and lung) and three nonrespiratory (liver, heart, and bladder) tissues and analyzed for DNA adducts -by 32P-postlabeling assay under conditions capable of detecting low levels of diverse aromatic/hydrophobic adducts. ata showed that the total DNA adducts in the lung, heart, trachea, and larynx were increased by 10- to 20-fold in the smoke-exposed group. ive-fold increase was observed in the bladder tissue, but differences were not present in the liver DNA of control and smoke-exposed groups. hese data suggest selective formation of DNA adducts In the tissues.