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HIGHLY SENSITIVE BIOASSAYS FOR EVALUATING AIRBORNE MUTAGENS INDOORS
Citation:
Goto, S., O. Endo, H. Matsushita, Y. Takagi, AND J. Lewtas. HIGHLY SENSITIVE BIOASSAYS FOR EVALUATING AIRBORNE MUTAGENS INDOORS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/D-90/165 (NTIS PB91132985).
Description:
The standard mutagenicity bioassays that are readily applied to the valuation of outdoor air samples collected by high volume samplers are not efficiently sensitive to measure the mutagenicity of low volume air samples collected indoors. wo microsuspension mutation assays using various strains of Salmonella typhimurium were evaluated for the assessment of indoor air pollution. ne assay used highly sensitive YG strains of bacteria in a histidine reversion assay. he other was a forward mutation assay detecting azaguanine resistant mutants in the TM677 strain. o decrease the required sample mass in the forward mutation assay, the volume in the preincubation step was decreased 10-fold to produce an ultra microsuspension assay. y applying these assays to indoor air samples, their usefulness was confirmed in investigating the effect of smoking, ventilation or air conditioning.
