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GENOTOXICITY OF 2-AMINO-6-N-HYDROXYADENINE (AHA) TO MOUSE LYMPHOMA AND CHO CELLS
Citation:
Moore, M., K. Harrington-Brock, L. Parker, C. Doerr, AND J. Hozier. GENOTOXICITY OF 2-AMINO-6-N-HYDROXYADENINE (AHA) TO MOUSE LYMPHOMA AND CHO CELLS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-91/213.
Description:
2-Amino-6-hydroxyadenine (AHA) was evaluated in L5178Y/TK+/--3.7.2C mouse lymphoma cells for mutation at tk, hgprt, and the Ouabain r loci, as well as for gross aberrations and induction of micronuclei. n addition, AMA was evaluated for its ability to induce mutants in CHO cells. MA was found to induce mutation under all of the test marker selections. he mutants were primarily large colonies, although a few small colonies were also induced, particularly at the higher concentrations, preliminary cytogenetic analysis of AMA-treated mouse lymphoma cells indicated that some gross aberrations but not micronuclei were induced. ore complete cytogenetic analysis will be performed. he 20 small-colony tk mutants evaluated by banded karyotype indicate that only a small fraction (2 of 20) showed chromosome 11 abnormalities. rom these studies, it appears that AMA may be one of a very few chemicals that is capable of inducing multi-locus point mutations, with only slight clastogenic activity. articularly at the higher concentrations, some of the mutants may contain multi-locus point mutations that result in slow growth.