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COMPARISON OF DNA ADDUCTS FROM COMPLEX MIXTURE EXPOSURES IN VARIOUS HUMAN TISSUES AND EXPERIMENTAL SYSTEMS
Citation:
Lewtas, J., R. Everson, B. Hulka, T. Wilcosky, W. Kozumbo, C. Thompson, M. George, L. Dobias, R. Sram, X. Li, AND J. Gallagher. COMPARISON OF DNA ADDUCTS FROM COMPLEX MIXTURE EXPOSURES IN VARIOUS HUMAN TISSUES AND EXPERIMENTAL SYSTEMS. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-93/357 (NTIS PB93228831).
Description:
DNA adducts derived from complex mixtures of polycyclic aromatic compounds emitted from tobacco smoke are compared to industrial pollution sources (e.g., coke ovens and aluminum, smelters), smoky coal burning, and urban air pollution. xposures to coke oven emissions and smoky coal, both potent rodent skin tumor initiators and lung carcinogens in humans, result in comparatively high levels of DNA adducts compared to tobacco smoke in the in-vitro calf thymus DNA model system, in cultured lymphocytes and the mouse skin assay. sing tobacco smoke as a model in human studies, we have compared relative DNA adduct levels detected in blood lymphocytes, placental tissue, bronchioalveolar lung lavage cells, sperm, and autopsy tissues of smokers and nonsmokers. dduct levels in DNA isolated from smokers were highest in human heart and lung tissue; with smaller but detectable differences in placental tissue and lung lavage cells. omparison of the DNA adduct levels resulting from human exposure to different complex mixtures show that emissions from coke ovens, aluminum smelters and smoky coal result in higher DNA adduct levels than tobacco smoke exposure. hese studies suggest that humans exposed to complex combustion mixtures will have higher DNA adduct levels in target cells (e.g., lung) as compared to nontarget cells (e.g., lymphocytes) and that the adduct levels will be dependent on the genotoxic and DNA adduct forming potency of the mixture.