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NASAL LAVAGE ANTIOXIDANTS IN GUINEA PIGS, RATS AND MICE
Citation:
Norwood, J., K. Crissman, AND G. Hatch. NASAL LAVAGE ANTIOXIDANTS IN GUINEA PIGS, RATS AND MICE. U.S. Environmental Protection Agency, Washington, D.C., EPA/600/J-95/086.
Description:
A new nasal lavage technique was used to compare the washout curves and total lavagable amounts (per kg body wt) of protein, ascorbate, glutathione and uric acid in guinea pigs, rats and mice. Washout curves were usually observed with sequential lavage volumes of saline of 1.0 ml in guinea pigs and rats and 0.5 ml in mice. Mice were found to have 2.0 times more lavagable protein than guinea pigs, and guinea pigs had about twice the amount as rats. There was undetectable lysozyme in all three species. Mice had about 2.0 times more AH2 in NL than guinea pigs, which had about 2.0 times more than rats. Glutathione was also much higher (10%) in mice than in the other species. Assay of nasal lavage antioxidants may serve as a biomarker for assessing effects of oxidant pollutants in the nose.